| Objective To investigate the influence of Glaucocalyxin A (GLA), a natural biological compound, on osteoarthritis (OA) chondrocytes.Methods Primary human osteoarthritis chondrocytes were divided into 5 groups according to the establishment of inflammatory cell model in vitro and different application concentrations of GLA: Normal control group, in which no treatment was applied to the cells; inflammatory model group, in which chondrocytes were treated with 10 ng/mL interleukin-1β (IL-1β) for 24 h; gradient concentration of GLA (5, 10, 20 μM) + IL-1β groups, in which chondrocytes were treated with different concentrations (5, 10, 20 μM) of GLA for 2 h, and then treated with 10 ng/mL IL-1β for 24 h. The mRNA and protein expression of inflammation related factors was detected by qRT-PCR, Western blot and ELISA. The NF-κB signal pathway protein expression was detected by Western blot and immunofluorescence in each group.Results GLA could inhibit the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in chondrocytes induced by IL-1β, and reduce the release of nitric oxide (NO) and prostaglandin E2 (PGE2). At the same time, GLA could also inhibit the production of inflammatory cytokines such as TNF-α, IL-6 and IL-8 in chondrocytes induced by IL-1β. GLA also inhibited the expression of p-p65 and the nuclear translocation of p65 in chondrocytes, and the differences were statistically significant (all P<0.05).Conclusion GLA can alleviate the inflammatory response of OA chondrocytes, which suggests that GLA may be an effective drug for the treatment of OA. |
