| Objective To investigate the effect of miR-654-3p on the proliferation of osteosarcoma cells, predict its target genes and assess the intervention of target genes on the survival prognosis of osteosarcoma patients.Methods The GSE70367 dataset in the GEO database was selected for differential analysis to screen for aberrantly expressed miRNAs in osteosarcoma cells, and the expression of miR-654-3p in osteosarcoma cell lines 143B, MG-63, SAOS2 and HOS was detected by RT-qPCR. The target genes of miR-654-3p were predicted by TargetScan and miRmap database, and the binding of miR-654-3p to GMFB gene was verified using dual luciferase reporter gene assay. The effects of miR-654-3p and GMFB on the proliferation of MG-63 and HOS in osteosarcoma cells were analyzed by CCK8 assay and CFU assay. GMFB expression data and clinical information of sarcoma patients in the TCGA database were downloaded, and KM curves of the effect of GMFB on survival prognosis and column plots predicting 1-year, 3-year, and 5-year survival of sarcoma patients were plotted by the R package.Results The expression of miR-654-3p was significantly lower in osteosarcoma cell lines 143B, MG-63, SAOS2 and HOS compared to human normal osteoblasts hFOB1.19 (P<0.05). miR-654-3p bound to the GMFB gene and negatively regulated the expression of GMFB. The miR-654-3p mimics significantly inhibited the proliferation of osteosarcoma cells in vitro, while GMFB transfection was able to reverse the inhibitory effect (P<0.05). Overall survival was significantly lower in sarcoma patients with high GMFB expression than in patients with low expression (P<0.05), and 1-year, 3-year, and 5-year survival rates could be predicted by the nomogram.Conclusion miR-654-3p inhibits the proliferation of osteosarcoma cells by targeting the negative regulation of GMFB gene expression, and high GMFB expression is negatively correlated with the prognosis of sarcoma patients. |
