| Objective To understand the degradation cycle of new absorbable bone wax by animal experiments, and to study the biocompatibility between absorbable bone wax and bone tissue.Methods The bone implantation test was performed on 18 experimental sheep. The six sternums of each sheep were used as independent implantation sites. Two sites were randomly selected for hemostasis with the experimental product absorbable bone wax (experimental group) or the control product Johnson & Johnson bone wax (control group), and the remaining two sites were used as blank (blank group). Six experimental sheep were randomly killed at 2, 6, and 12 weeks after operation. The sternum was collected for HE sections for histologic evaluation and material degradation analysis. Toxic symptoms of 20 mice were observed by tail vein injection and intraperitoneal injection of sample extract and control. Six rabbits were used to observe the tissue reaction of erythema and edema at each injection site through intradermal injection of sample extract and control.Results Compared with the blank group, the experimental object was implanted on the sternal injury surface for 2, 6 and 12 weeks and showed no stimulation. Compared with the experimental group, the control group showed moderate stimulation after implantation for 6 and 12 weeks. The histologic response of the test device was less than that of the control. No fibrous tissue capsule was found during the degradation of absorbable bone wax, and the new bone grew obviously. At 12 weeks, the sample was basically degraded completely, the two broken ends of the new bone were connected, and the bone injury was repaired. In the acute toxicity test, the weight of the mice in each group increased steadily. In the stimulation test, the integral of the physiological saline of the sample was 0, and the score of corn oil was 0.11.Conclusion After 12 weeks, the absorbable bone wax can be completely absorbed by the bone. It is a safe, non-toxic, and hemostatic material for bone wound. |
