| Bone formation and bone remodeling involve the synergistic action of various cells. By culturing various in vitro cell models, a comprehensive understanding of the biological aspect of the osteogenic process has been advanced. Currently, bone tissue engineering research has recently become a hot topic, but due to the limited availability of primary human osteoblasts, in vitro cell models are gradually being widely used. MC3T3-E1 cells are pro-osteoblasts, and this cell line has great potential for studying cell therapy and tissue engineering of skeletal diseases and bone defects. In this paper, we describe the culture, identification and application of MC3T3-E1 cell osteogenesis model, further discuss the advantages and disadvantages of this model for simulating the osteogenesis process and present the outlook. |
