Voronoi仿生骨支架：孔隙率对其细胞黏附的影响及其流体动力学机制

作者	单位	邮编
卢腾^*	西安交通大学第二附属医院骨科，陕西西安，710004	710004
李杰	西安交通大学第二附属医院骨科，陕西西安，710004	710004
王栋	西安交通大学第二附属医院骨科，陕西西安，710004	710004
李浩鹏^*	西安交通大学第二附属医院骨科，陕西西安，710004	710004

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中文摘要:

目的探讨孔隙率对Voronoi仿生支架内部间充质干细胞（mesenchymal stem cell, MSC）黏附效率的影响及其流体动力学机制。方法构建流体动力学模型，比较不同孔隙率（60%、70%、80%、90%）Voronoi支架渗透率、流体流速分布、流体运动路径的差异。基于离散相模型的流体动力学模型进一步模拟MSC在支架内部运动与黏附行为，对比不同孔隙率Voronoi支架内部MSC的黏附效率。结果 60%、70%、80%、90%孔隙率Voronoi支架，流体出入口ΔP（压力差）分别为0.280 Pa、0.177 Pa、0.140 Pa、0.102 Pa；渗透率分别为0.374 2 m²、0.593 2 m²、0.747 0 m²、1.034 0 m²；支架内部低流速流体占比逐渐升高，而高流速流体占比逐渐降低；流体运动路径复杂程度逐渐降低；MSC黏附效率分别为10.9%、14.7%、9.0%、8.5%。结论 70%左右孔隙率能够最大化Voronoi支架的MSC黏附效率，有利于支架内部的MSC黏附增殖与成骨分化。这是由于相比于其他大小孔隙率，70%孔隙率下Voronoi支架内部流体运动路径的复杂程度和流体流速对MSC的拖拽力更有利于MSC的深部迁移和表面黏附。

英文摘要:

Objective To investigate the effect of porosity on the adhesion efficiency of mesenchymal stem cells (MSCs) within Voronoi bionic scaffolds, as well as the underlying hydrodynamic mechanisms.Methods A hydrodynamic model was constructed to analyse differences in permeability, fluid velocity distribution and fluid flow paths in Voronoi scaffolds with different porosities (60%, 70%, 80% and 90%). Based on the discrete phase model (DPM), the movement and adhesion behavior of the MSCs within the scaffolds was simulated and the adhesion efficiency of MSCs in scaffolds with different porosities was compared.Results For Voronoi scaffolds with porosities of 60%, 70%, 80%, and 90%, the fluid inlet-outlet pressure differences (ΔP) were 0.280 Pa, 0.177 Pa, 0.140 Pa, and 0.102 Pa, respectively, and the permeabilities were 0.374 2 m², 0.593 2 m², 0.747 0 m², and 1.034 0 m², respectively. The proportion of low-velocity fluid within the scaffolds gradually increased, while the proportion of high-velocity fluid gradually decreased. The complexity of fluid flow paths progressively reduced. The MSC adhesion efficiencies were 10.9%, 14.7%, 9.0%, and 8.5%, respectively.Conclusion A porosity of approximately 70% maximizes the MSC adhesion efficiency in Voronoi scaffolds, facilitating MSC adhesion, proliferation, and osteogenic differentiation within the scaffold. Compared to scaffolds with other porosity levels, the 70% porosity Voronoi scaffold exhibits fluid flow paths and fluid velocity-induced drag forces that are more conducive to the deep migration and surface adhesion of MSCs.

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