| 载有二甲双胍的含铜海藻酸钠水凝胶在糖尿病足溃疡治疗中的应用潜力研究 |
| Copper alginate hydrogel containing metformin for potential use in the treatment of diabetic foot ulcers |
| 投稿时间：2025-01-14 |
| DOI：10.3969/j.issn.1672-5972.2025.06.002 |
| 中文关键词: 海藻酸钠 铜 二甲双胍 糖尿病足 抗菌 |
| 英文关键词:Sodium alginate Copper Metformin Diabetic foot Antibacterial |
| 基金项目:荆州市联合科研基金项目（2024LHY17）；长江大学医学部科技援藏“医卫人才培养计划项目”（2023YZ07）；荆州第一人民医院博士科研启动基金项目（20122dif01） |
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| 中文摘要: |
| 目的 基于海藻酸钠（SA）与铜离子（Cu2+）构建了具有抗菌性能和良好生物相容性的SA-Cu水凝胶，并将二甲双胍（MH）负载其中，探讨其在糖尿病足溃疡创面治疗中的潜力。方法 将海藻酸钠溶解于去离子水中，得到浓度为3%（w/v）的海藻酸钠溶液，随后加入0.1 mol/L氯化铜溶液中制备含铜海藻酸钠（SA-Cu）水凝胶。通过扫描电子显微镜（SEM）观察水凝胶的微观结构，并采用共培养实验评估SA-Cu水凝胶的抗菌性能。利用CCK-8试剂盒检测SA-Cu对细胞的影响，并通过活/死染色法评估其细胞相容性。确定MH的最佳浓度后，将其负载于SA-Cu水凝胶中，形成SA-Cu-MH水凝胶。采用傅立叶变换红外光谱（FTIR）分析材料之间的化学结合，最后通过溶胀率和药物释放实验评估MH的缓释性能。结果 SA-Cu水凝胶具有均匀的孔隙结构，并展现出优异的抗菌性能，能有效抑制金黄色葡萄球菌和大肠杆菌的生长。CCK-8实验表明，SA-Cu水凝胶对细胞具有较低的细胞毒性，显示出良好的生物相容性。活/死染色结果进一步确认了其对细胞的低毒性及支持细胞生长的能力。MH的最佳浓度经CCK-8确认后，将其负载于SA-Cu水凝胶中。FTIR分析显示SA-Cu-MH之间形成了化学结合。溶胀率和药物释放实验结果表明，MH能够在SA-Cu-MH水凝胶中缓慢且持续地释放。结论 SA-Cu水凝胶具有优异的抗菌性和生物相容性。负载MH后的SA-Cu-MH水凝胶展示了良好的药物缓释性能，具有作为糖尿病足溃疡治疗材料的潜力。 |
| 英文摘要: |
| Objective To construct an antimicrobial and biocompatible sodium alginate (SA)-based SA-Cu hydrogel loaded with metformin (MH) for the treatment of diabetic foot ulcers.Methods Sodium alginate was dissolved in deionized water to obtain a 3% (w/v) sodium alginate solution, followed by the addition of a 0.1 mol/L copper chloride solution to prepare the SA-Cu hydrogel. The microstructure of the hydrogel was observed using scanning electron microscopy (SEM), and the antimicrobial properties of the SA-Cu hydrogel were evaluated through co-culture experiments. The effects of the SA-Cu hydrogel on cells were assessed using the CCK-8 assay, and its cytocompatibility was evaluated by live/dead staining. After determining the optimal concentration of metformin (MH), it was loaded into the SA-Cu hydrogel to form the SA-Cu-MH hydrogel. Fourier transform infrared spectroscopy (FTIR) was used to analyze the chemical bonding between the materials, and swelling rate and drug release experiments were performed to evaluate the sustained release properties of MH.Results The SA-Cu hydrogel exhibited a uniform porous structure and demonstrated excellent antimicrobial properties, effectively inhibiting the growth of Staphylococcus aureus and Escherichia coli. The CCK-8 assay indicated that the SA-Cu hydrogel had low cytotoxicity, showing good biocompatibility. Live/dead staining further confirmed its low toxicity and ability to support cell growth. After determining the optimal concentration of MH via the CCK-8 assay, MH was successfully loaded into the SA-Cu hydrogel. FTIR analysis revealed chemical bonding between the SA, Cu and MH components. Swelling rate and drug release experiments revealed that MH was released slowly and continuously from the SA-Cu-MH hydrogel.Conclusion The SA-Cu hydrogel has excellent antimicrobial properties and biocompatibility. The SA-Cu-MH hydrogel, loaded with MH, demonstrated good drug release performance and holds potential as a therapeutic material for diabetic foot ulcers. |
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