| [Abstract] Objective The establishment of collagen induced arthritis model, Discussion different concentrations of IL-17A neutralizing antibody to CIA serum inflammatory cytokines in mice. Methods 48 male SPF C57BL/6 mice were randomly divided into 4 groups: A（Normal group）group; B（The CIA and Saline Group）group; C（The CIA and high concentration group）group; D（The CIA and low concentration group）group. Use of chicken type II collagen fluorine's complete adjuvant emulsion preparation of antigen tail more subcutaneous injection; Two weeks later, Primary immune with the same method for the second immunization, intraperitoneal inject neutralizing anti -murine interleukin-17 antibody or physiological saline after CIA mice models were induced; persistent observation and data record of the change of the general situation, mice weight，arthritis index and thickness of fat in two weeks, testing the CIA mice serum TNF-a, IL-6, IL-17 expression level. Results C, D and B compared of TNF-a, IL-17 and IL-6 expression level decreased significantly; neutralizing anti-murine interleukin-17 antibody of the high concentration group were lower than low concentrations in serum inflammatory cytokine expression level, the difference was statistically significant （P <0.05）. Conclusion Neutralizes interleukin-17 antibody can significantly reduce Inflammatory cytokine of the CIA mice model in serum TNF-a, IL-17, IL-6 expression level, And the highest concentration group improved joint function more significant than the low concentration group, The mechanism may be involved in regulating Th17 mediated by cytokines-osteoclasts immune system, Inhibit the inflammatory cytokine expression, Further affect osteoclast factor to achieve. |
