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复合磷酸钙颗粒溶解性能测试研究
The study on dissolution testing of Calcium phosphate composite particles
  
DOI:
中文关键词:  磷酸钙  溶解行为  电感耦合等离子体质谱法
英文关键词:Calcium phosphate  Dissolution behavior  ICP-MS
基金项目:
作者单位
马春宝 李沅 景明 樊铂  
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中文摘要:
      目的 研究复合磷酸钙颗粒(HA:TCP=3:7)在TRIS缓冲溶液中的体外溶解特性。方法 配制一系列浓度逐级递增的Ca2+标准溶液,绘制Ca2+离子溶液的工作曲线。将复合磷酸钙颗粒(HA:TCP=3:7)置于pH值为7.4的TRIS缓冲溶液中浸泡,每小时取样一次(初始阶段每3分钟取样一次),用高速离心技术提取上层清液,采用电感耦合等离子体质谱法测定各时间点提取液的Ca2+浓度,绘制Ca2+浓度对浸泡时间的溶解曲线。结果① Ca2+离子溶液的工作曲线的线性方程为y=4043.9x+23783,相关系数R为0.9999,方法检出限为0.0063 μg/mL,定量限为0.021 μg/mL。②浸泡后最初的0.5 h以内,Ca2+浓度随时间延长急剧升高,0.5 h处有明显转折,0.5 h至20 h,Ca2+浓度仍随时间增加而升高,但是增加的速率较前0.5 h明显下降,20 h以后溶解达到基本平衡。③复合磷酸钙颗粒的溶解过程分为3个阶段,首先是Ca2+离子浓度快速升高的溶解过程,第二阶段是Ca2+离子溶解与HA或CaHPO4·2H2O沉积的竞争过程,第三阶段是Ca2+离子浓度不再继续增加的稳定过程。结论 复合磷酸钙颗粒(HA:TCP=3:7)在TRIS缓冲溶液中的溶解具有一定的规律性,24 h后溶出的Ca2+离子浓度在1200 μg/L左右。
英文摘要:
      Objective To study the dissolution characteristics of calcium phosphate composite particles (HA:TCP=3:7) in TRIS buffer solution. Methods A series of standard Ca2+ solutions with increasing concentration are prepared, and the working curve of Ca2+ ion solution was drawn. The composite calcium phosphate particles (HA:TCP=3:7) in the pH value for 7.4 TRIS buffer solution, sampling once per hour (initial samples were collected every 3 min), extracted from the supernatant by high speed centrifugation, measured at each time point of the concentration of Ca2+ extraction liquid by inductively coupled plasma mass spectrometry. The dissolution curve of Ca2+ concentration on soaking time was drawn. Results ①The linear equation of the working curve of Ca2+ ionic solution is y=4043.9x+23783, and the correlation coefficient R is 0.9999. The detection limit is 0.0063 μg/mL, and the quantitative limit is 0.021 μg/mL. ② After the initial immersion within 0.5 h, the concentration of Ca2+ increased with time dramatically, marked a turning point at 0.5 h. From 0.5 h to 2 h, the concentration of Ca2+ ion increased with time, but the rate of increase decreased obviously compared with the first 0.5 h. After 20 h, the dissolution reached the basic balance. ③ The dissolution process of calcium phosphate composite particles can be divided into 3 stages, the first is the dissolution process of Ca2+ ion concentration increased rapidly, the second stage is the competition process of Ca2+ dissolution with HA or CaHPO4·2H2O deposition, the third stage is the stable process of Ca2+ ion concentration will no longer increase. Conclusion The dissolution of composite calcium phosphate particles (HA:TCP=3:7) in TRIS buffer solution has a certain regularity, and the concentration of Ca2+ ions dissolved in 24h is about 1200 μg/L.
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