Objective To establish a simple and feasible animal model of rabbit adjuvant arthritis and to identify it. Methods The rabbits were injected complete Freund's adjuvan (CFA) to establish the model of adjuvant arthritis. Adjuvant arthritis(AA) rabbits the perimeters of the hind toe were measured before and after modeling. The changements of soft tissue and bone were assessed by X-rays. Pathological changes were observed by HE staining method in synovial tissue. Results Before modeling, the perimeters of the toe in four groups showed great difference compared with normal group (P<0.05). Through X ray pictures, compared with the normal group, the edema of model group soft tissue was more serious, joint space loss or even obscured, articular surface is not smooth. During the extraction process, it was found that the model rabbit joint cavity had obvious effusion, and the model rabbit toe was dissected with obvious microvascular hyperplasia and inflammation. After the synovial tissue HE staining, the synovial cells in the normal group showed a beaded pattern arrangement, and the synovial cells in the model group were disordered and edema. Conclusion The animal model of rabbit adjuvant arthritis is successfully established, the method is simple and the success rate is high, which provides a good experimental animal model for further research. |