基于选区激光熔融技术制备的多孔钛合金支架的力学性能及成骨能力评价*

目的评价选区激光熔融（selective laser melting，SLM）技术制备的多孔钛合金支架的力学性能及成骨能力。方法通过SLM技术制备两种多孔钛合金（Ti6-Al4-V）支架，分别为均一多孔组和复合多孔组，简称均一组与复合组，并对其力学性能、体外细胞相容性和体内骨整合能力进行评价。结果与传统锻件相比，多孔钛合金支架的弹性模量更接近人体正常骨组织，且复合组的弹性模量高于均一组，差异具有统计学意义（P＜0.05）。细胞相容性方面，随着时间的推移，间充质干细胞数量在两组多孔支架上均有增加，但复合组钛合金支架在5 d和7 d的细胞数量高于均一组（P＜0.05）。该组在成骨分化实验的7 d也具有更高的ALP活性（P＜0.05）。诱导14 d后两组ALP活性无差异。体内植入实验表明，3个月时，复合组的骨体积分数与均一组比较差异无统计学意义（P＞0.05）。结论本研究表明，基于SLM制备的多孔钛合金支架弹性模量适宜，具有骨整合能力，适合做骨缺损修复材料。

英文摘要:

Objective To evaluate the mechanical properties and osteogenesis ability of porous titanium alloy scaffolds manufactured by selective laser melting (SLM) technique. Methods Two kinds of porous titanium alloy (Ti6-Al4-V) scaffolds were fabricated by SLM technique, which were single pore structure group and compound pore structure group, and their mechanical properties, in vitro cell compatibility and in vivo osteogenesis ability were evaluated. Results Compared with the traditional forging technology, the elastic modulus of the porous titanium scaffolds were closer to the human cancellous bone and the elastic modulus of the compound group was higher than that of the single group, which was statistically significant (P<0.05). In terms of cell compatibility, the number of mesenchymal stem cells on the 5th and 7th day of the compound group was significantly higher than the single group(P<0.05). This group also had higher ALP activity on the 7th day of the osteogenic differentiation experiment (P<0.05), but there was no difference in ALP activity between the two groups after 14 days of induction. In vivo experiments showed that at 3 months, the bone volume fraction of the compound group was not significantly different from that of the single group (P>0.05). Conclusion This study shows that the porous titanium alloy scaffolds manufactured by SLM is suitable for bone defect repair materials.

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