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HA涂层对3D打印钛合金材料安全性的影响
The effect of HA coating on the safety of 3D printing titanium alloy material
  
DOI:
中文关键词:  有HA涂层  无HA涂层  安全性  3D打印  钛合金
英文关键词:HA coating  Non-HA coating  Safety  3D printing  Titanium alloy
基金项目:
作者单位
杜晓丹1# 孙聪慧1# 赵丹妹1 姜爱莉2 陈丹丹1*  
通讯作者:杜晓丹1# 孙聪慧1# 赵丹妹1 姜爱莉2 陈丹丹1*    
摘要点击次数: 45
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中文摘要:
      目的 探究羟基磷灰石(HA)涂层对3D打印钛合金材料生物安全性的影响。方法 将3D打印钛合金材料分为有HA涂层材料和无HA涂层材料两个样品组。分别进行细胞毒性、刺激、急性毒性、溶血、回复突变、小鼠淋巴瘤试验和细胞致癌试验,将两种材料的生物安全性评价进行对比,观察羟基磷灰石(HA)涂层对3D打印钛合金髋臼杯材料的生物安全性的影响。结果 在细胞毒性试验中,有HA涂层组的细胞存活率为(117±6)%,无HA涂层组的细胞存活率为(106±14)%,均大于75%;在刺激试验中,两种材料生理盐水浸提液组的积分均为0,玉米油浸提液组的积分均为0.06;在急性毒性试验中,各组小鼠体重均稳步增长;在溶血试验中,有HA涂层材料的溶血率为0.125%,无HA涂层材料的溶血率为-0.257%;在Ames试验中,两种材料的回复突变菌落数均在正常值范围内;在小鼠淋巴瘤试验中,各剂量组的突变频率MF均大于或小于阴性对照组,且稍微高于阴性对照组的剂量组均不超过126×10-6。结论 两种材料均不具有细胞毒性、急性毒性、刺激和遗传毒性,同时也证明了两种材料都不具有溶血作用。有HA涂层材料和无HA涂层材料均表现出良好的生物安全性,证明HA涂层材料是安全可靠的,这也为未来开钛合金材料通过添加涂层改性提供了安全依据。
英文摘要:
      Objective To explore the impact of hydroxyapatite (HA) coating on the biosafety of 3D printing titanium alloy materials. Methods 3D printing titanium alloy materials were divided into two sample groups: HA coated materials and non-HA coated materials. Cytotoxicity, irritation, acute toxicity, hemolysis, reverse mutation, mouse lymphoma test and cell carcinogenesis test were carried out respectively. The biosafety evaluation of the two materials was compared to observe the effect of hydroxyapatite (HA) coating on the biosafety of 3D printed titanium alloy acetabular cup material. Results In the cytotoxicity test, the cell survival rate of the HA coated group was (117±6)%, and the cell survival rate of the non-HA coated group was (106±14)%, both of which were greater than 75%; in the stimulation test, the integral of the physiological saline of the two materials were 0, the integral of corn oil of the two materials were 0.06; in the acute toxicity, the weight of the mice in each group increased steadily; in the hemolysis test, the hemolysis rate of the HA coated material was 0.125%, and the hemolysis rate of the non-HA coated group was -0.257%; in the Ames test, the numbers of back-mutated colonies of the two materials were within the normal range; in the mouse lymphoma test, the mutation frequency MF of each dose group was greater than or less than that of the negative control group, and the slightly dose higher than the negative control group did not exceed 126×10-6. Conclusion The experiment results proved that the two materials do not have cytotoxicity, acute toxicity, irritation and genetic toxicity, and also proved that the two materials do not have hemolytic effect. Both HA coated materials and non-HA coated materials showed good biological safety, which proves that HA coated materials are safe and reliable, and it also provides a way for future titaniumalloymaterials to bemodified by adding coatings safety basis.
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