| 力学性能可控的甲基丙烯酸化明胶（GelMA）人工软骨性能及软骨/骨表界面结合研究 |
| Mechanical property tunable GelMA artificial cartilage and cartilage/bone interface |
| 投稿时间：2021-11-29 修订日期：2022-03-24 |
| DOI：10.3969/j.issn.1672-5972.2022.05.002 |
| 中文关键词: 骨关节炎 软骨损伤 软骨组织工程 甲基丙烯酸酐化明胶水凝胶 软骨/骨表界面结合 |
| 英文关键词:Osteoarthritis Cartilage injury Cartilage tissue engineering Methacrylate anhydride gelatin hydrogel Cartilage/bone surface interface |
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| 中文摘要: |
| 目的 探寻优化骨表面力学匹配的软骨修复材料，并探讨软骨修复材料与骨结合的表界面性能，为软骨修复材料的设计及制备提供依据。方法 以明胶和甲基丙烯酸酐为原料，在明胶分子中引入双键结构，使用紫外光交联的方式制备甲基丙烯酸化明胶（GelMA）水凝胶。运用扫描电子显微镜、万能力学试验机分析水凝胶的形貌、孔径大小和力学性能；体外降解实验、体外拉伸实验评估水凝胶的降解和拉伸特性；黏附能力定性实验、三维立体光学显微镜和扫描电子显微镜评估水凝胶的黏附能力；细胞增殖测试和Live/Dead染色测定细胞活性和毒性。探讨了不同紫外光照时间下GelMA水凝胶的理化性能及软骨/骨材料结合的表界面结合性能，对性能适宜的修复材料进行了配比、力学优化。结果 紫外光照（UV）交联时间为1 min时水凝胶孔隙最大（110.25±6.51）μm，孔隙率高达（45.24±2.78）%；12 h时水凝胶的平衡溶胀比达（148.43±3.84）%；28 d失重率为（17.40±2.38）wt%；水凝胶的拉伸性能随紫外光照时间延长而逐渐增加；GelMA水凝胶对骨髓间充质干细胞增殖无明显抑制作用；水凝胶软骨修复材料与骨的结合良好，可黏附于仿生骨材料表面。结论 紫外光照交联时间1 min时GelMA水凝胶的吸水速率和平衡溶胀比最佳，降解速快，拉伸性能与天然软骨结构类似，具有良好的生物相容性，与骨修复材料力学匹配，该力学性能可控的GelMA水凝胶软骨修复材料为软骨/骨的连接及性能匹配提供了依据。 |
| 英文摘要: |
| Objective To explore the optimization of bone surface mechanics matching cartilage repair materials, and explore the surface interface properties of cartilage repair materials and bone bonding, to provide a basis for the design and preparation of cartilage repair materials.Methods The gelatin and methacrylate anhydride were used as raw materials, and GelMA was synthesized, where a double bond structure was introduced into the gelatin molecule, and the GelMA hydrogel was prepared by UV crosslinking. The morphology, pore size and mechanical properties of hydrogel were analyzed by scanning electron microscope and universal mechanical testing machine. The degradation and tensile properties of hydrogels were evaluated by in vitro degradation test and in vitro tensile test. The adhesive ability of hydrogel was evaluated by qualitative experiment, three-dimensional optical microscope and scanning electron microscope. Cell proliferation test and Live/Dead staining were used to determine cell viability and toxicity. The physicochemical properties of GelMA hydrogel under different UV time and the interface bonding properties of cartilage/bone material were discussed, the appropriate repair materials were proportioned and mechanical optimized.Results When UV crosslinking time was 1 min, the hydrogel pores reached the maximum (110.25±6.51) μm and the porosity reached (45.24±2.78)%. The equilibrium swelling ratio of hydrogel reached (148.43±3.84)% at 12 h. The weight loss rate of 28 d was (17.40±2.38) wt%. The tensile properties of hydrogels increased with increasing UV exposure time. GelMA hydrogel did not inhibit the proliferation of BMSCs. The hydrogel cartilage repair material was well combined with bone and can adhere to the surface of biomimetic bone material.Conclusion When the UV light crosslinking time is 1 min, GelMA hydrogel has the best water absorption rate and equilibrium swelling ratio, and degrades quickly. Its tensile properties are similar to natural cartilage structure, and it has good biocompatibility and is mechanically matched with bone repair materials. The GelMA hydrogel cartilage repair material with controllable mechanical properties provides a basis for cartilage/bone connection and property matching. |
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