| IRAK4-IN-1对SD大鼠炎症软骨细胞炎症和衰老表型的影响 |
| Effects of IRAK4-IN-1 on inflammation and senescence phenotype in inflammatory chondrocytes of rat |
| 投稿时间：2022-04-14 修订日期：2022-08-31 |
| DOI：10.3969/j.issn.1672-5972.2022.06.004 |
| 中文关键词: IRAK4-IN-1 骨关节炎 炎症 衰老 P65/NF-ĸB |
| 英文关键词:IRAK4-IN-1 Osteoarthritis Inflammation Aging P65/NF-ĸB |
| 基金项目:湖北省武汉市市卫健委项目（WX19C18） |
|
| 摘要点击次数: 182 |
| 全文下载次数: 159 |
| 中文摘要: |
| 目的 研究IRAK4-IN-1对炎症软骨细胞的炎症、分解代谢、衰老表型的影响及其作用机制。方法 利用免疫荧光染色和甲苯胺蓝染色法鉴定SD大鼠的乳鼠原代软骨细胞，使用CCK8法筛选IRAK4-IN-1的安全浓度范围，本实验设置空白对照组（Control）、IL-1β刺激组、IL-1β+IRAK4-IN-1（1、5、10 μM）组，通过蛋白免疫印迹法检测炎症因子、基质降解酶、衰老标志物及P65/NF-ĸB通路蛋白的表达。结果 IRAK4-IN-1显著抑制IL-1β刺激下炎症因子iNOS、COX2，基质降解酶MMP13、ADAMTS5以及衰老标志物P16蛋白的表达，此外P65/NF-ĸB通路的激活也受到显著抑制。结论 IRAK4-IN-1在炎症软骨细胞中具有抗炎、抑制分解代谢和衰老的作用，其可能是通过抑制P65/NF-ĸB通路发挥炎症调控作用。 |
| 英文摘要: |
| Objective To explore the effects of IRAK4-IN-1 on inflammation, catabolism and senescence phenotype in inflammatory chondrocytes and the underlying mechanism.Methods Primary chondrocytes of SD rats were identified by immunofluorescence and toluidine blue staining, and the safe concentration range of IRAK4-IN-1 was screened by CCK8 method. There were control group, IL-1β stimulated group and IL-1β+IRAK4-IN-1 (1, 5, 10 μM) group in the study. Western blot assay was used to detect the protein expression of inflammatory factors, matrix degrading enzymes, aging markers and P65/NF-ĸB pathway protein.Results IRAK4-IN-1 significantly inhibited the expression of inflammatory factors iNOS, COX2, matrix degrading enzyme MMP13, ADAMTS5 and aging marker P16 protein under the stimulation of IL-1β. In addition, activation of P65/NF-ĸB pathway was also repressed.Conclusion IRAK4-IN-1 had anti-inflammatory, anti-catabolism and aging-ameliorating effects, and inhibition of P65/NF-ĸB pathway might play an important part in the inflammation regulation. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
